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interleukin 1 receptor associated kinase  (Proteintech)


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    Proteintech interleukin 1 receptor associated kinase
    Interleukin 1 Receptor Associated Kinase, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 59 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/interleukin+1+receptor+associated+kinase/IRAK1+Antibody/pmc12822004-94-57-62
    Average 95 stars, based on 59 article reviews
    interleukin 1 receptor associated kinase - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Immunohistochemistry:

    Article Title: Sodium butyrate modulates ocular surface microbiome and attenuates inflammation of meibomian gland dysfunction in ApoE -/- mice.
    Article Snippet: .. Immunohistochemistry (IHC) staining was conducted to identify cells in conjunctivae and MGs positive for CD3 (ab16669, Abcam, 1:500), CD4 (ab183685, Abcam, 1:500), toll-like receptor 4 (TLR4, 19811-1-AP, Proteintech, 1:200), toll/interleukin-1 receptor domain-containing adaptor protein (TIRAP, ab17218, Abcam, 1:200), myeloid differentiation primary response 88 (MyD88, PA5-19918, Invitrogen, 1:250), tumor necrosis factor receptor-associated factor 6 (TRAF6, ab137452, Abcam, 1:200), interleukin-1 receptor-associated kinase (IRAK1,10478-2-AP, Proteintech, 1:200), IkappaB kinase beta (IKKβ, ab124957, Abcam, 1:200), nuclear factor-kappa B (NF-κB) p65 (ab16502, Abcam, 1:200), and interferon-gamma (IFN-γ, bs-0480R, Bioss, 1:100), IL-17A antibody (26163-1-AP, Proteintech, 1:500), and inducible nitric oxide synthase (iNOS, 80517-1-RR, Proteintech, 1:200). .. Sections were then treated with a secondary goat anti-rabbit antibody (ab205718, Abcam, 1:1000) and stained with 3,3’-diaminobenzidine (DAB, ZSGB-BIO, Beijing, China).

    Article Title: Sodium butyrate modulates ocular surface microbiome and attenuates inflammation of meibomian gland dysfunction in ApoE −/− mice
    Article Snippet: .. Immunohistochemistry (IHC) staining was conducted to identify cells in conjunctivae and MGs positive for CD3 (ab16669, Abcam, 1:500), CD4 (ab183685, Abcam, 1:500), toll-like receptor 4 (TLR4, 19811-1-AP, Proteintech, 1:200), toll/interleukin-1 receptor domain-containing adaptor protein (TIRAP, ab17218, Abcam, 1:200), myeloid differentiation primary response 88 (MyD88, PA5-19918, Invitrogen, 1:250), tumor necrosis factor receptor-associated factor 6 (TRAF6, ab137452, Abcam, 1:200), interleukin-1 receptor-associated kinase (IRAK1, 10478-2-AP, Proteintech, 1:200), IkappaB kinase beta (IKKβ, ab124957, Abcam, 1:200), nuclear factor-kappa B (NF-κB) p65 (ab16502, Abcam, 1:200), and interferon-gamma (IFN-γ, bs-0480R, Bioss, 1:100), IL-17A antibody (26163-1-AP, Proteintech, 1:500), and inducible nitric oxide synthase (iNOS, 80517-1-RR, Proteintech, 1:200). .. Sections were then treated with a secondary goat anti-rabbit antibody (ab205718, Abcam, 1:1000) and stained with 3,3′-diaminobenzidine (DAB, ZSGB-BIO, Beijing, China).

    Staining:

    Article Title: Sodium butyrate modulates ocular surface microbiome and attenuates inflammation of meibomian gland dysfunction in ApoE -/- mice.
    Article Snippet: .. Immunohistochemistry (IHC) staining was conducted to identify cells in conjunctivae and MGs positive for CD3 (ab16669, Abcam, 1:500), CD4 (ab183685, Abcam, 1:500), toll-like receptor 4 (TLR4, 19811-1-AP, Proteintech, 1:200), toll/interleukin-1 receptor domain-containing adaptor protein (TIRAP, ab17218, Abcam, 1:200), myeloid differentiation primary response 88 (MyD88, PA5-19918, Invitrogen, 1:250), tumor necrosis factor receptor-associated factor 6 (TRAF6, ab137452, Abcam, 1:200), interleukin-1 receptor-associated kinase (IRAK1,10478-2-AP, Proteintech, 1:200), IkappaB kinase beta (IKKβ, ab124957, Abcam, 1:200), nuclear factor-kappa B (NF-κB) p65 (ab16502, Abcam, 1:200), and interferon-gamma (IFN-γ, bs-0480R, Bioss, 1:100), IL-17A antibody (26163-1-AP, Proteintech, 1:500), and inducible nitric oxide synthase (iNOS, 80517-1-RR, Proteintech, 1:200). .. Sections were then treated with a secondary goat anti-rabbit antibody (ab205718, Abcam, 1:1000) and stained with 3,3’-diaminobenzidine (DAB, ZSGB-BIO, Beijing, China).

    Article Title: Sodium butyrate modulates ocular surface microbiome and attenuates inflammation of meibomian gland dysfunction in ApoE −/− mice
    Article Snippet: .. Immunohistochemistry (IHC) staining was conducted to identify cells in conjunctivae and MGs positive for CD3 (ab16669, Abcam, 1:500), CD4 (ab183685, Abcam, 1:500), toll-like receptor 4 (TLR4, 19811-1-AP, Proteintech, 1:200), toll/interleukin-1 receptor domain-containing adaptor protein (TIRAP, ab17218, Abcam, 1:200), myeloid differentiation primary response 88 (MyD88, PA5-19918, Invitrogen, 1:250), tumor necrosis factor receptor-associated factor 6 (TRAF6, ab137452, Abcam, 1:200), interleukin-1 receptor-associated kinase (IRAK1, 10478-2-AP, Proteintech, 1:200), IkappaB kinase beta (IKKβ, ab124957, Abcam, 1:200), nuclear factor-kappa B (NF-κB) p65 (ab16502, Abcam, 1:200), and interferon-gamma (IFN-γ, bs-0480R, Bioss, 1:100), IL-17A antibody (26163-1-AP, Proteintech, 1:500), and inducible nitric oxide synthase (iNOS, 80517-1-RR, Proteintech, 1:200). .. Sections were then treated with a secondary goat anti-rabbit antibody (ab205718, Abcam, 1:1000) and stained with 3,3′-diaminobenzidine (DAB, ZSGB-BIO, Beijing, China).



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    BA inhibits the production and expression of inflammatory molecules in LPS-stimulated BV-2 microglia. The effects of BA on NO production (a), iNOS activity (b), <t>IL-1</t> β (c), and PGE2 (d) production in LPS-stimulated BV-2 cells. BV-2 cells were incubated for 1 h in the presence of the indicated concentrations of BA (2.5-22.5 μ M). Then, the cells were stimulated with or without LPS (0.1 μ g/mL); the production of NO and iNOS (incubated for 12 h) and IL-1 β and PGE2 (incubated for 24 h) was determined by ELISA kit. MiR-155 expression (e) was detected by miRNA real-time PCR kit, BV-2 was pretreated with BA (2.5-22.5 μ M) for 1 h followed by LPS stimulation for 12 h, and then, RT-PCR was performed. Results were presented as mean ± SD ( n = 3). ### P < 0.001 vs. control and ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001 vs. LPS-treated cells.
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    Image Search Results


    BA inhibits the production and expression of inflammatory molecules in LPS-stimulated BV-2 microglia. The effects of BA on NO production (a), iNOS activity (b), IL-1 β (c), and PGE2 (d) production in LPS-stimulated BV-2 cells. BV-2 cells were incubated for 1 h in the presence of the indicated concentrations of BA (2.5-22.5 μ M). Then, the cells were stimulated with or without LPS (0.1 μ g/mL); the production of NO and iNOS (incubated for 12 h) and IL-1 β and PGE2 (incubated for 24 h) was determined by ELISA kit. MiR-155 expression (e) was detected by miRNA real-time PCR kit, BV-2 was pretreated with BA (2.5-22.5 μ M) for 1 h followed by LPS stimulation for 12 h, and then, RT-PCR was performed. Results were presented as mean ± SD ( n = 3). ### P < 0.001 vs. control and ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001 vs. LPS-treated cells.

    Journal: BioMed Research International

    Article Title: Baicalin Mitigates the Neuroinflammation through the TLR4/MyD88/NF- κ B and MAPK Pathways in LPS-Stimulated BV-2 Microglia

    doi: 10.1155/2022/3263446

    Figure Lengend Snippet: BA inhibits the production and expression of inflammatory molecules in LPS-stimulated BV-2 microglia. The effects of BA on NO production (a), iNOS activity (b), IL-1 β (c), and PGE2 (d) production in LPS-stimulated BV-2 cells. BV-2 cells were incubated for 1 h in the presence of the indicated concentrations of BA (2.5-22.5 μ M). Then, the cells were stimulated with or without LPS (0.1 μ g/mL); the production of NO and iNOS (incubated for 12 h) and IL-1 β and PGE2 (incubated for 24 h) was determined by ELISA kit. MiR-155 expression (e) was detected by miRNA real-time PCR kit, BV-2 was pretreated with BA (2.5-22.5 μ M) for 1 h followed by LPS stimulation for 12 h, and then, RT-PCR was performed. Results were presented as mean ± SD ( n = 3). ### P < 0.001 vs. control and ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001 vs. LPS-treated cells.

    Article Snippet: Interleukin-1 receptor-associated kinase 1/4 inhibitor (IRAK1/4 inhibitor), TLR4, P-I κ B α , and I κ B α antibodies were purchased from Santa Cruz Biotechnology.

    Techniques: Expressing, Activity Assay, Incubation, Enzyme-linked Immunosorbent Assay, miRNA RT, Reverse Transcription Polymerase Chain Reaction

    Mechanism of BA mitigating the neuroinflammation responses in LPS-stimulated BV-2 microglia. BA mitigates the neuroinflammation responses in LPS-stimulated BV-2 through the TLR4/MyD88/NF- κ B and MAPK pathway. BA inhibits the expression of miR-155 and release of inflammatory mediator (NO, PGE2, and IL-1 β ).

    Journal: BioMed Research International

    Article Title: Baicalin Mitigates the Neuroinflammation through the TLR4/MyD88/NF- κ B and MAPK Pathways in LPS-Stimulated BV-2 Microglia

    doi: 10.1155/2022/3263446

    Figure Lengend Snippet: Mechanism of BA mitigating the neuroinflammation responses in LPS-stimulated BV-2 microglia. BA mitigates the neuroinflammation responses in LPS-stimulated BV-2 through the TLR4/MyD88/NF- κ B and MAPK pathway. BA inhibits the expression of miR-155 and release of inflammatory mediator (NO, PGE2, and IL-1 β ).

    Article Snippet: Interleukin-1 receptor-associated kinase 1/4 inhibitor (IRAK1/4 inhibitor), TLR4, P-I κ B α , and I κ B α antibodies were purchased from Santa Cruz Biotechnology.

    Techniques: Expressing