interleukin 1 receptor associated kinase  (Proteintech)

Journal: BioMed Research International

Article Title: Baicalin Mitigates the Neuroinflammation through the TLR4/MyD88/NF- κ B and MAPK Pathways in LPS-Stimulated BV-2 Microglia

doi: 10.1155/2022/3263446

Figure Lengend Snippet: BA inhibits the production and expression of inflammatory molecules in LPS-stimulated BV-2 microglia. The effects of BA on NO production (a), iNOS activity (b), IL-1 β (c), and PGE2 (d) production in LPS-stimulated BV-2 cells. BV-2 cells were incubated for 1 h in the presence of the indicated concentrations of BA (2.5-22.5 μ M). Then, the cells were stimulated with or without LPS (0.1 μ g/mL); the production of NO and iNOS (incubated for 12 h) and IL-1 β and PGE2 (incubated for 24 h) was determined by ELISA kit. MiR-155 expression (e) was detected by miRNA real-time PCR kit, BV-2 was pretreated with BA (2.5-22.5 μ M) for 1 h followed by LPS stimulation for 12 h, and then, RT-PCR was performed. Results were presented as mean ± SD ( n = 3). ### P < 0.001 vs. control and ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001 vs. LPS-treated cells.

Article Snippet: Interleukin-1 receptor-associated kinase 1/4 inhibitor (IRAK1/4 inhibitor), TLR4, P-I κ B α , and I κ B α antibodies were purchased from Santa Cruz Biotechnology.

Techniques: Expressing, Activity Assay, Incubation, Enzyme-linked Immunosorbent Assay, miRNA RT, Reverse Transcription Polymerase Chain Reaction

Journal: BioMed Research International

Article Title: Baicalin Mitigates the Neuroinflammation through the TLR4/MyD88/NF- κ B and MAPK Pathways in LPS-Stimulated BV-2 Microglia

doi: 10.1155/2022/3263446

Figure Lengend Snippet: Mechanism of BA mitigating the neuroinflammation responses in LPS-stimulated BV-2 microglia. BA mitigates the neuroinflammation responses in LPS-stimulated BV-2 through the TLR4/MyD88/NF- κ B and MAPK pathway. BA inhibits the expression of miR-155 and release of inflammatory mediator (NO, PGE2, and IL-1 β ).

Article Snippet: Interleukin-1 receptor-associated kinase 1/4 inhibitor (IRAK1/4 inhibitor), TLR4, P-I κ B α , and I κ B α antibodies were purchased from Santa Cruz Biotechnology.

Techniques: Expressing